scPlotRegion#
scPlotRegion plots signal from individual cells in a given genomic region as a heatmap (in the style of a trackplot),
togather with the summary profiles (pseudobulk signal) on top.
usage:
scPlotRegion -i INPUT_binned.h5ad --region CHROM:START-END -o OUTPUT_PREFIX
Input/Output options#
- --input, -i
sincei-generated input file in .h5ad format.
- --outFilePrefix, -o
Prefix for output file names.
Plot Options#
- --region, -r
Genomic region in CHROM:START-END format
- --mode, -m
Possible choices: sum, mean
How to aggregate signal for plotting the summary profiles (pseudobulk) on top. (default: 'sum')
- --signalMin
Minimum value for pseudobulk track plot (default: minimum signal in the region)
- --signalMax
Maximum value for pseudobulk summary profile (default: maximum signal in the region)
- --hmapMin
Minimum value for single-cell heatmap signal (default: minimum signal in the region)
- --hmapMax
Maximum value for single-cell heatmap signal (default: maximum signal in the region)
- --summaryPlotColor
Color for the pseudobulk summary profile plot (default: 'red')
- --colorMap
Colormap for heatmap. Must be a valid matplotlib colormap entry (default: 'Reds')
- --figsize
Figure size in inches (<width>, <height>; (default: (14, 8)))
- --dpi
DPI for output PNG (default: 300)
- --outFileFormat
Possible choices: png, pdf, svg, eps, plotly
image format type. If given, this option overrides the image format inferred from the suffix of the outFilePrefix
Other options#
- --verbose, -v
Set to see processing messages.
- -V, --version
Print the program version and exit.